Vivaspin 20, 5,000 MWCO PES, 12pc., $ 219,45

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High flow rates due to PES membranes Sample recovery in concentrate > 90% Vertical membrane for fast concentration Compatible with swing-out and fixed-angle rotors Safe concentration due to dead-stop reservoir Easy concentrate removal by pipetting Volume graduation for easy reading 100-fold concentration due to vertical twin membranes Additional option for pressure filtration Description Vivaspin® 20 centrifugal concentrators are designed to offer increased volume flexibility and performance. Vivaspin® 20 can process samples up to 20 ml in swing-out rotors or 14 ml in fixed-angle rotors for 50 ml centrifuge tubes. The units are equipped with vertically mounted twin membranes for unparalleled filtration speed and concentration factors over 100-fold. The remaining volume can be easily read from the printed scale and the modified dead stop reservoir facilitates direct pipetting of the concentrate. For greater process flexibility, Vivaspin® 20 offers special accessories and innovative procedures. For one-step desalting of the sample after concentration, a diafiltration cup filled with buffer is placed on the concentrator and centrifuged a second time to remove 98 % of the salts. Compared to the traditional procedure (refill/recentrifuge), several steps are saved. Pressure filtration: If no suitable centrifuge is available or only a single sample is processed, Vivaspin® 20 can be filled with up to 15 ml of sample and used as a 'stand alone' unit under gas pressure. Pressure centrifugation: For even faster concentration, pressure filtration can be combined with centrifugation. This unique process is particularly suitable for difficult, e.g. viscous samples such as serum, for low process temperatures or generally when minimum process times are essential. Rotor compatibility Please note: Vivaspin® 20 ultrafiltration units can be operated in rotors suitable for Falcon 50 units with a vertical bottom, such as Beckman Allegra 25R with TS-5. 1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus / Sorvall) 75006445 swing-out rotor with 75006441 buckets and adaptors for 50 ml Falcon conical bottom tubes. Performance characteristics Time required to concentrate up to 30x [min] at 20°C and solute recovery % Mode Centrifuge Centrifuge Bench-top Press-fuge Rotor Swing-out rotor 25° Fixed-angle rotor Pressure Swing-out rotor Sart volume 20 ml 14 ml 10 ml 10 ml Min./Rec. Min./Rec. Min./Rec. Cytochrome c 0.25 mg/ml (12,400 MW) 3,000 MWCO PES 110 / 97 % 180 / 96 % 60 / 96 % - / - BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 23 / 99 % 29 / 99 % 50 / 98 % 14 / 98 % 10. 000 MWCO PES 16 / 98 % 17 / 98 % 32 / 97 % 8 / 97 % 30,000 MWCO PES 13 / 98 % 15 / 98 % 32 / 97 % 8 / 97 % IgG 0.25 mg/ml (160. 000 MW) 30,000 MWCO PES 27 / 97 % 20 / 95 % 46 / 94 % 13 / 97 % 50,000 MWCO PES 27 / 96 % 22 / 95 % 46 / 93 % 13 / 96 % 100,000 MWCO PES 25 / 91 % 20 / 90 % 42 / 88 % 12 / 94 % Latex beads 0.004 % in DMEM 10 % FCS (0.055 µm) 300. 000 MWCO PES 20 / 99 % 35 / 99 % 10 / 99 % - / - Latex beads 0.004 % in DMEM 10 % FCS (0.24 µm) 1,000,000 MWCO PES 4 / 99 % 12 / 99 % 4 / 99 % - / - Yeast 1.0 mg/ml (S. Cerevisiae) 0.2 µm PES 15 / 95 % 5 / 95 % 20 / 95 % 2 / 95 % VIVASPIN 20 5,000 MWCO PES 12/BOX, Vivaspin 20, 5,000 MWCO PES, 12pc

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